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Plateau technique Xylomic : Génomique et phénotypage des arbres
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Nouveau projet de mobilité Trees4Future

le mardi 02 septembre 2014

Nous accueillons à partir du 2 septembre Cindy VERDU, de Gembloux Agro-Bio Tech (Université de Liège), pour une durée de 3 mois. Son travail s'inscrit dans le cadre du projet DIVERSBLOC : étude de la diversité génétique du Robinier faux-acacia (Robinia pseudoacacia L.)

Nouveau projet de mobilité Trees4FutureRobinia pseudoacacia

Résumé du projet de recherche :

The use of renewable resources as an alternative to fossil fuels has become a priority. Producing biomass and developing “green chemistry” from forest tree species require the development of suitable and competitive selected genotypes ready to meet the challenges of global change. In this context, the black locust, Robinia pseudoacacia L., is a very promising species for which Gembloux Agro-Bio Tech has already developed an expertise.
Black locust is a tree species native of the Appalachian Mountains and the Ozark Plateau in the east of the United States [1]. It was introduced in France at the beginning of the 17th century and subsequently spread throughout Europe and temperate regions of the world. Firstly use for its horticultural properties [2], interests of black locust became numerous: as plantation for stabilizing and revegetating disturbed areas [3], as timber for wood production [2], honeybee [4], feed [5] and more recently for the production of molecules with high added value [6] and biomass for energy production [7].
Its potential of improvement in traditional breeding program has already been demonstrated in Hungary, the largest producer and exporter of wood and seeds of black locust in Europe [8]. However, the genetic improvement of woody species using traditional methods can take between 20 to 30 years. Thanks to genomic selection, we are now able to reduce this delay and quickly respond to adverse environmental changes (depletion of agricultural land, climate change, limited water and energy resources).

The development of a genomic selection program must first be based on a genetic diversity study. Despite the growing interest of black locust in Europe, very few studies on its genetic diversity are available, and currently none in Belgium. In particular, getting knowledge about the genetic resources available in the native area of the species could be useful for future breeding programs. Moreover, to efficiently develop genetic diversity studies and breeding programs, new suitable molecular markers must be designed. SNP (Single Nucleotide Polymorphism) markers are particularly well adapted to these purposes: abundant, homogeneously distributed in the genome, highly transferable and less sensitive to genotyping bias [9].

Génotypage

[1]: Kennedy, J.M., Geographic variation in black locust (Robinia pseudoacacia L.). University of Georgia ed. 1983. 132. [2]: DeGomez, T. and M.R. Wagner, Culture and use of black locust. HortTechnology, 2001. 11: p. 279-288. [3]: Boring, L.R. and W.T. Swank, Symbiotic nitrogen fixation in regenerating black locust (Robinia pseudoacacia L.) stands. Forest Science, 1984. 30(2): p. 528-537. [4]: Persano Oddo, L. and R. Piro, Main European unifloral honeys: descriptive sheets. Apidology, 2004. 35: p. S38-S81. [5]: Baertsche, S.R., M.T. Yokoyama, and J.W. Hanover, Short rotation, hardwood tree biomass as potential ruminant feed-chemical composition, nylon bag ruminal degradation and ensilement of selected species. Journal of Animal Science, 1986. 63: p. 2028-2043. [6]: Millspaugh, C.F., American medicinal plants, ed. D.P. Inc. 1974, New York. 832. [7]: Redei, K., I. Veperdi, M. Tome, and P. Soares, Black locust (Robinia pseudoacacia L.) short-rotation energy crop in Hungary: A review. Silva Lusitana, 2010. 18(2): p. 217-223. [8]: Redei, K., Z. Osvath-Bujtas, and I. Veperdi, Black locust (Robinia pseudoacacia L.) improvement in Hungary: A review. Acta Silvatica et Lignaria Hungarica, 2008. 4: p. 127-132. [9]: Hauser, L., M. Baird, R. Hilborn, L.W. Seeb, and J.E. Seeb, An empirical comparison of SNPs and microsatellites for parentage and kinship assignment in a wild sockeye salmon (Oncorhynchus nerka) population. Molecular Ecology Resources, 2011. 11(Suppl. 1): p. 150-161.



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